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gse13294 geo affymetrix hg u133  (Thermo Fisher)


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    Thermo Fisher gse13294 geo affymetrix hg u133
    Gse13294 Geo Affymetrix Hg U133, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gse13294 geo affymetrix hg u133/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    gse13294 geo affymetrix hg u133 - by Bioz Stars, 2026-06
    94/100 stars

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    GCS is involved in modulating gene expression of ovarian cancer cells in response to dactinomycin treatments. Cells of NCI/ADR-RES/Mock (Mock), NCI/ADR-RES/GCS (GCS), and NCI/ADR-RES/asGCS (asGCS) ovarian cancer cell lines were treated with 25 nM dactinomycin (DAC) in 5% FBS for 24 h. The mRNA levels of genes were analyzed by using Affymetrix GeneChip ® Human Genome U133 Plus 2.0 and Transcriptome Analysis Console (TAC 4.0). ( A ), differential gene-expression profiles of NCI/ADR-RES cells in response to DAC treatments. Differential expression of probed genes was identified as those having transcript levels 2-fold higher (up-regulated) or 2-fold lower (down-regulated) for the cells with treatment than those of comparator cells. ( B ), Overlaps of gene profiles of NCI/ADR-RES cells with treatments. GCS-responsive genes (overlap AB areas) are identified as those exhibiting their expression levels up-regulated in ADR-RES/GCS cells versus down-regulated in ADR/RES/asGCS cells (vice versa) treated with vehicle (left) or DAC (right), respectively.

    Journal: International Journal of Molecular Sciences

    Article Title: Glucosylceramide Synthase, a Key Enzyme in Sphingolipid Metabolism, Regulates Expression of Genes Accounting for Cancer Drug Resistance

    doi: 10.3390/ijms26115112

    Figure Lengend Snippet: GCS is involved in modulating gene expression of ovarian cancer cells in response to dactinomycin treatments. Cells of NCI/ADR-RES/Mock (Mock), NCI/ADR-RES/GCS (GCS), and NCI/ADR-RES/asGCS (asGCS) ovarian cancer cell lines were treated with 25 nM dactinomycin (DAC) in 5% FBS for 24 h. The mRNA levels of genes were analyzed by using Affymetrix GeneChip ® Human Genome U133 Plus 2.0 and Transcriptome Analysis Console (TAC 4.0). ( A ), differential gene-expression profiles of NCI/ADR-RES cells in response to DAC treatments. Differential expression of probed genes was identified as those having transcript levels 2-fold higher (up-regulated) or 2-fold lower (down-regulated) for the cells with treatment than those of comparator cells. ( B ), Overlaps of gene profiles of NCI/ADR-RES cells with treatments. GCS-responsive genes (overlap AB areas) are identified as those exhibiting their expression levels up-regulated in ADR-RES/GCS cells versus down-regulated in ADR/RES/asGCS cells (vice versa) treated with vehicle (left) or DAC (right), respectively.

    Article Snippet: The GeneChip TM Human Genome U133 Plus 2.0 Array (Affymetrix; catalog 900466) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Gene Expression, Quantitative Proteomics, Expressing